| Titre : | Introduction and expression of an insect proteinase inhibitor in alfalfa (Medicago sativa L.) |
| Auteurs : | J. Thomas ; C. Wasmann ; C. ECHT ; R. DUNN ; H. BOHNERT ; T. MCCOY |
| Type de document : | article/chapitre/communication |
| Année de publication : | 1994 |
| Format : | vol. 14 no. 1 ; 31-36 |
| Langues: | = Anglais |
| Mots-clés: | INSECTICIDE ; INHIBITEUR ENZYME ; MANDUCA SEXTA ; PROTEINASES ; REGENERATION ; EXPRESSION GENIQUE ; TRANSFORMATION GENETIQUE ; MEDICAGO SATIVA ; RESISTANCE ; GENE HYBRIDE ; LUTTE ANTIDEPREDATEUR ; PLANTE FOURRAGERE ; GENE ; SPHINGIDAE ; LEPIDOPTERA ; INSECTA ; ARTHROPODA ; INVERTEBRATA ; HYDROLASES ; ENZYME ; LEGUMINOSAE ; DICOTYLEDONES ; ANGIOSPERMAE ; SPERMATOPHYTA ; PLANTE TRANSGENIQUE ; Genome ; Transfert de gene ; Medicago sativa ; Leguminosae ; Manduca sexta |
| Résumé : | As one approach to alleviating the need for insecticide spraying, our objective is to express protein insecticides in transgenic alfalta. To initiate these studies, a cDNA encoding the protease inhibitor (PI) antielastase from Manduca sexta was placed under the control of the CaMV 35S promoter, inserted into pAN 70, and transferred into leaf and petiole sections of alfalfa (Medicago sativa L.) using Agrobacterium tumefaciens mediated gene transfer. Transformation rates were 10% of all explants exposed to Agrobacterium. More than 1000 transgenic plants containing the PI have been recovered. Transgenic plants were initially identified when leaf explants from the regenerated plants formed callus in the presence of 50 mug/ml kanamycin, and subsequently the presence of the PI gene was confirmed by southern analysis |
| Source : | Plant cell reports |
Exemplaires (1)
| Centre | Localisation | Section | Cote | Statut | Disponibilité | Département |
|---|---|---|---|---|---|---|
| Poitou-Charentes | ERIST Poitou-Charentes - Lusignan | Base Aphide | A4C1E2 | Empruntable | Disponible pour le prêt |
