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Titre :
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Genetic Diversity Within a Potato Leafhopper (Homoptera: Cicadellidae) Population Infesting Alfalfa
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Auteurs :
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D. Skinner ;
R. Camacho
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Type de document :
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article/chapitre/communication
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Année de publication :
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1995
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Format :
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35-42
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Langues:
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= Anglais
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Mots-clés:
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Genetique des populations
;
Biologie moleculaire
;
RAPD
;
Deplacement
;
Empoasca fabae
;
Cicadellidae
;
Medicago sativa
;
Leguminosae
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Résumé :
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Random Amplified Polymorphic DNA (RAPD) markers were used to examine genetic diversity within a potato leafhopper population infesting an alfalfa field in Kansas. Eight primers were used to generate a total of 52 RAPD fragments; 46 were polymorphic. All 48 potato leafhopper individuals examined had unique RAPD fragment profiles. Calculations of genetic parameters revealed little evidence of genetic linkage disequilibrium, suggesting the population was panmictic. The average genetic distance (proportion of loci at which two insects differed) was 0.29 +/- 0.11, and the estimated average level of heterozygosity per locus was 0.26 +/- 0.17. The high level of genetic variation within a potato leafhopper population infesting an alfalfa field appears to be maintained through random mating.
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Source :
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Journal of the Kansas Entomological Society - 0022-8567, vol. 68, n° 1
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