| Titre : | Microbial aspects of environmental changes after wastewater discharges in a river |
| Titre original: | Aspects microbiens des changements environnnementaux liés aux rejets d'eaux usées en rivière |
| Auteurs : | B. Montuelle ; M. Torre ; CEMAGREF LYON QELY ; J. Pernelle ; CEMAGREF BORDEAUX QEBX ; CEMAGREF ANTONY QEAN |
| Type de document : | article/chapitre/communication |
| Année de publication : | 1998 |
| Format : | p. 1250-1253 |
| Note générale : |
Sigle : CEMAGREF Sigle : QELY Sigle : QEBX Sigle : QEAN Sigle : EDS Diffusion tous publics |
| Langues: | = Anglais |
| Catégories : | |
| Mots-clés: | CHARENTE COURS D'EAU ; SEINE COURS D'EAU ; BACTERIE ; IMPACT SUR L'ENVIRONNEMENT ; REJET EN COURS D'EAU ; STATION D'EPURATION ; EAU USEE |
| Résumé : |
Les rejets d'eaux usées apportent dans les milieux récepteurs, en plus de charges organiques et minérales, des charges bactériennes parfois élevées. Plusieurs études de laboratoire ou de terrain ont abordé ceproblème dans le but de contribuer à l'évaluation du devenir des bactéries de station d'épuration après leur rejet en rivière et après le stress physiologique qu'elles subissent. Wastewater treatment plants discharges bring about in places large disturbances to aquatic environments: supply of chemicals compounds such as carbon (more or less biodegradable), but also, sometimes in great quantity nitrogen and phosphorus. These disturbances especially affect the microbial and the biogeochemical processes. Bacteria issued from allochtonous sources could also have an influence on the autochtonous bacterial populations and their activity in the microbial loop. Especially wastewater treatment plants (WTP) outfalls have a high bacterial load but, in spite of their marked polluting effects on rivers and without considering the sanitary aspects, our knowledges of WTP discharges impact are fragmentary. Some works on site (Charente river and Seine river, France) and at the laboratory (microcosms) have studied this problem following the main purpose of the estimation of the fate of WTP's bacteria after their discharge in rivers and the physiological stress they undergo. We have used several techniques, separately or associated, for field or laboratory studies, on freshwater, sediments and WTP discharges (treated water) samples - systems for identification of bacteria by specific metabolic types: API Strips (API-NFT; API 20NE,..) and Biolog (microtiter plates for Gram- and Gram +). - a SDS-PAGE proteins electrophoresis technique has been recently used for aquatic microbiology studies. This technique allows detection of 30 to 50 proteic bands per bacterial strains and is proved to be discriminant at the species. Works carry on to improve it.The obtained informations are sufficient for comparison of unknown strains to the electrophoretic profile of reference strains (collections strains: ATCC for exemple). This technique is optimized by the use of image analysis system which analyzes the gels and allows a precise determination of homology level between different strains (a specific software "Gel Analyzer" has been developped for MacIntosh microcomputer (Cemagref - Graftek patent). - immunofluorecence techniques, using polyclonal antibodies, are used for studying the biodiversity of the genus Nitrobacter and its changes in polluted rivers. Amongst the broad serotypes diversity of the this nitrifying bacterial genus, we have used 6 serotypes that are related to different species of Nitrobacter : N. wynogradskyi (serotypes AG, DE11, W and DE30), N. hamburgensis (serotype LL) and new genomic species not yet named (serotype X14). - microbial activities measurements: biodegradation of organic carbon, enzymes activities, ... |
| Source : | Verh. Internat. Verein. Limnol., vol n° 26 |
Exemplaires (1)
| Centre | Localisation | Section | Cote | Statut | Disponibilité | Département |
|---|---|---|---|---|---|---|
| Lyon | Villeurbanne | Articles/chapitres/communications | DOC/CQELY.6687 | Consultable sur place | Exclu du prêt |
