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Titre :
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Genetic relationships among biotypes of Bemisia tabaci (Hemiptera : Aleyrodidae) based on AFLP analysis
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Auteurs :
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M. Cervera ;
J. Cabezas ;
B. Simon ;
J. Martinezzapater ;
F. Beitia ;
J. Cenis
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Type de document :
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article/chapitre/communication
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Année de publication :
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2000
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Format :
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391-396
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Langues:
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= Anglais
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Mots-clés:
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AFLP
;
biotype
;
repartition geographique
;
biologie moleculaire
;
Bemisia tabaci
;
Aleyrodidae
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Résumé :
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Genetic similarities between 13 samples belonging to nine reference biotypes and two field populations of Bemisia tabaci (Gennadius), one field population of B. medinae Gomez-Menor and another of B. afer Priesner & Hosny, were evaluated using amplified fragment length polymorphism (AFLP) markers. The results indicate that B. tabaci biotypes can be grouped together with a minimum similarity coefficient of 0.32 and separated from the two other species with a similarity coefficient of 0.07. Bemisia tabaci biotypes were grouped in four clusters which comprised: (i) Near East and Indian subcontinent biotypes; (ii) B and Q biotypes plus a Nigerian population from cowpea; (iii) New World A biotype; and (iv) S biotype and a Nigerian population from cassava. These results were consistent with a previous grouping of biotypes based on RAPD-PCR analysis. The AFLP assay allowed the scoring of a total of 354 polymorphic bands in two reaction events with the use of two primer combinations.
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Source :
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Bulletin of Entomological Research - 0007-4853, vol. 90, n° 5
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