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33 recherche sur le mot-clé
'Buchnera' 
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J. Wernegreen ; N. Moran | 1999Buchnera, the bacterial endosymbionts of aphids, undergo severe population bottlenecks during maternal transmission through their hosts. Previous studies suggest an increased effect of drift within these strictly asexual, small populations, resu[...]
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B. Sabater ; R. Vanham ; D. Martineztorres ; F. Silva ; A. Latorre ; A. Moya | 2001Aphids maintain an obligate, endosymbiotic association with Buchnera sp., a bacterium closely related to Escherichia coli. Bacteria are housed in specialized cells of organ-like structures called bacteriomes in the hemocoel of the aphid and are [...]
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T. Wilkinson ; A. Douglas | 1998The symbiotic bacteria Buchnera in aphids are borne in cells, called bacteriocytes, in the insect haemocoel. The number and median volume of bacteriocytes in pre-reproductive adult insects varied significantly among 14 parthenogenetic clones of [...]
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T. Soler ; A. Latorre ; B. Sabater ; F. Silva | 2000
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N. Humphreys ; A. Douglas | 1997The population of symbiotic Buchnera bacteria in parthenogenetic females of the yea aphid Acyrthosiphon pisum mas determined by quantitative hybridization of a DNA probe (gro ESL) to aphid homogenates. The aphids bore 1 x 10(7) to 2 x 10(7) bact[...]
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H. Charles ; H. Ishikawa | 1999The genome of Buchnera, an endosymbiotic bacterium of the pea aphid Acyrthosiphon pisum, was characterized by pulse-field gel electrophoresis (PFGE) as a circular DNA molecule of 657 kb. The enzymes I-Ceu I, Cpo I, Apa I, Sma I, Nae I, Sac II, M[...]
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A. Nakabachi ; H. Ishikawa | 1999Differential c DNA display and quantitative RT-PCR suggested that the riboflavin synthase complex of the aphid endosymbiont, Buchnera, is active only when the symbiotic system is maintained and well organized in young hosts. Since this finding s[...]
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K. Komaki ; H. Ishikawa | 2000Buchnera, endosymbiotic bacteria of aphids possess many genomic copies per cell. In this study, we estimated genomic copy number per Buchnera cell from host insects at various developmental stages and of two different morphs, apterae and alatae,[...]
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A. Nakabachi ; H. Ishikawa | 1997Bacteriocytes harbouring Buchnera endosymbionts were isolated from young and old aphids, Acyrthosiphon pisum, and their m RNA populations were examined by the differential c DNA display and quantitative reverse transcriptase-polymerase chain rea[...]
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C. Montllor ; A. Maxmen ; A. Purcell | 20021. Natural populations of pea aphids in California contain at least two facultative bacterial secondary symbionts (pea aphid secondary symbiont, PASS, or pea aphid rickettsia, PAR) in a range of frequencies throughout the state. 2. Two pea aphid[...]
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J. Sandstrom ; N. Moran | 1999Aphids harbour intracellular symbionts (Buchnera) that provide their host with amino acids present in low amounts in their diet, phloem sap. To find out the extent to which aphids depend on their symbionts for synthesis of individual essential a[...]
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D. Adams ; A. Douglas | 1997To explore the effect of rearing-plant species on the contribution of the symbiotic bacterium, Buchnera, to aphid performance. larvae of Aphis fabae that contained the bacteria (symbiotic aphids) and larvae experimentally deprived of the bacteri[...]
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J. Sandstrom ; J. Russell ; J. White ; N. Moran | 2001Many insect groups have obligate associations with primary endosymbionts: mutualistic bacteria that are maternally transmitted and derived from an ancient infection. Often, the same insects are hosts to 'secondary' bacterial symbionts which are [...]
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T. Wilkinson ; H. Ishikawa | 2000The symbiotic bacteria Buchnera contribute to the nutrition of pea aphids, Acyrthosiphon pisum, through the provision of essential amino acids which are lacking in the diet. However, chemically defined diets, containing nutritionally adequate am[...]
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K. Komaki ; H. Ishikawa | 1999Although Buchnera, the endosymbiotic bacteria of aphids, are close relatives of Escherichia coli, their genome size is only a seventh that of E. coli. In this study, we estimated the genomic copy number of Buchnera by dot-blot hybridization and [...]
