Titre :
|
Use of aphid stylectomy and RT-PCR for the detection of transporter m RNAs in sieve elements
|
Titre original:
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Use of aphid stylectomy and RT-PCR for the detection of transporter m RNAs in sieve elements
|
Auteurs :
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C. Doeringsaad ;
H. Newbury ;
J. Bale ;
J. Pritchard
|
Type de document :
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article/chapitre/communication
|
Année de publication :
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2002
|
Format :
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631-637
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Langues:
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= Anglais
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Mots-clés:
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aphid
;
barley
;
phloem
;
RT-PCR
;
stylectomy
;
transporter
;
Phloeme
;
Seve
;
Composition biochimique
;
Stylectomie
;
ARN
;
Cellule compagne
;
Tube crible
;
Hordeum vulgare
;
Gramineae
;
Rhopalosiphum padi
;
Aphididae
|
Résumé :
|
Unmodified samples of barley (Hordeum vulgare) sieve tube sap have been obtained by severing the stylets (stylectomy) of feeding aphids and collecting the exuding liquid. Primers were designed to direct the amplification of a series of specific c DNAs encoding barley proteins selected because of their significance in sieve tube function. m RNA encoding the H+/sucrose co-transporter SUT1, a putative aquaporin and the H+/ATPase PPA1 were detected in sieve tube sap. These m RNA species appear to be present at very low concentrations. m RNA encoding the potassium transporter HAK1 could not be detected. The results strongly suggest that some m RNA species are imported into sieve elements, which are enucleate, from neighbouring companion cells.
|
Source :
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Journal of Experimental Botany VL 53 NO 369
|