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Résumé :
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The influence of Acyrthosiphon pisum's bacterial endosymbiosis on the biogenesis of the aphid peculiar triglycerides (TG) was investigated. We present a convenient way to obtain aposymbiotic aphids, extensive NMR data on the four main aphid-specific TG (1,3-di-myristoyl 2-hexanoyl, octanoyl, sorboyl or octatrienoyl glycerides) and an HPLC method to analyse these TG. The main results are: (1) aphid symbiotes are not responsible for the biosynthesis of the unusual sorbic and octatrienoic acid containing TG; (2) diet-reared aphids, although much fatter than plant-reared individuals, are not altered in their triglyceride profile, ruling out plant-specific dietary requirements for the synthesis of the odd TG; and (3) the sorbic acid containing TG, already described in cornicular secretions of aphids, was found to be strongly tissue-specific between normal fat-body and mature cornicular fat-cells ( 35% in the latter).
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