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T. Sasaki ; T. Aoki ; H. Hayashi ; H. Ishikawa | 1990
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Y. Hongoh ; H. Ishikawa | 1994Prior to migratory flight of alatiform aphids, their total volume of mycetocyte was greatly reduced. Since in this period they ingest little food, it is likely that they develop the flight muscles at the cost of their mycetocytes. In this period[...]
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A. Nakabachi ; H. Ishikawa | 1997Bacteriocytes harbouring Buchnera endosymbionts were isolated from young and old aphids, Acyrthosiphon pisum, and their m RNA populations were examined by the differential c DNA display and quantitative reverse transcriptase-polymerase chain rea[...]
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H. Harada ; H. Oyaizu ; Y. Kosako ; H. Ishikawa | 1997
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H. Harada ; H. Ishikawa | 1997
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K. Komaki ; H. Ishikawa | 2000Buchnera, endosymbiotic bacteria of aphids possess many genomic copies per cell. In this study, we estimated genomic copy number per Buchnera cell from host insects at various developmental stages and of two different morphs, apterae and alatae,[...]
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T. Sasaki ; H. Hayashi ; H. Ishikawa | 1991
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T. Wilkinson ; H. Ishikawa | 2000The symbiotic bacteria Buchnera contribute to the nutrition of pea aphids, Acyrthosiphon pisum, through the provision of essential amino acids which are lacking in the diet. However, chemically defined diets, containing nutritionally adequate am[...]
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K. Komaki ; H. Ishikawa | 1999Although Buchnera, the endosymbiotic bacteria of aphids, are close relatives of Escherichia coli, their genome size is only a seventh that of E. coli. In this study, we estimated the genomic copy number of Buchnera by dot-blot hybridization and [...]
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M. Kobayashi ; H. Ishikawa | 19941. Immunoblotting of proteins from indirect flight muscles of an alate aphid suggested that their breakdown subsequent to flight and feeding consists of ubiquitin-dependent proteolysis.2. While starvation of alatae prevented their flight muscles[...]
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M. Kobayashi ; H. Ishikawa | 1994We studied the changes in protein synthesis in the indirect flight muscles of alata of the pea aphid, Acyrthosiphon pisum during their development and degeneration subsequent to the final ecdysis, and obtained the following results. (1) While ov[...]
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T. Wilkinson ; H. Ishikawa | 2001All phloem-feeding Homoptera possess symbiotic microorganisms. Although the phylogenetic position and anatomical location of the micro- organisms differ, the underlying theme of the symbiosis is the same; the microorganisms improve the nutrition[...]
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H. Charles ; H. Ishikawa | 1999The genome of Buchnera, an endosymbiotic bacterium of the pea aphid Acyrthosiphon pisum, was characterized by pulse-field gel electrophoresis (PFGE) as a circular DNA molecule of 657 kb. The enzymes I-Ceu I, Cpo I, Apa I, Sma I, Nae I, Sac II, M[...]
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A. Nakabachi ; H. Ishikawa | 1999Differential c DNA display and quantitative RT-PCR suggested that the riboflavin synthase complex of the aphid endosymbiont, Buchnera, is active only when the symbiotic system is maintained and well organized in young hosts. Since this finding s[...]
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T. Wilkinson ; H. Ishikawa | 1999The failure of a nutritionally balanced diet to ameliorate the impact of symbiont disruption in the pea aphid Acyrthosiphon pisum (Harris) was investigated using two approaches. The assimilation of dietary nutrients by aphids was investigated us[...]
