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T. Wilkinson ; A. Douglas | 1995Pea aphids (Acyrthosiphon pisum) whose symbiotic bacteria were disrupted with the antibiotic chlortetracycline over the first 5 days after birth (i.e, aposymbiotic aphids), were able to feed from the host plant Vicia faba, as indicated by the re[...]
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T. Wilkinson ; D. Ashford ; J. Pritchard ; A. Douglas | 1997Pea aphids, Acyrthosiphon pisum, containing their symbiotic bacteria (untreated aphids) and experimentally deprived of their bacteria by treatment with the antibiotic rifampicin (antibiotic-treated aphids) were reared on the plant Vicia faba, Th[...]
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T. Wilkinson ; A. Douglas | 1998The symbiotic bacteria Buchnera in aphids are borne in cells, called bacteriocytes, in the insect haemocoel. The number and median volume of bacteriocytes in pre-reproductive adult insects varied significantly among 14 parthenogenetic clones of [...]
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T. Wilkinson ; H. Ishikawa | 2000The symbiotic bacteria Buchnera contribute to the nutrition of pea aphids, Acyrthosiphon pisum, through the provision of essential amino acids which are lacking in the diet. However, chemically defined diets, containing nutritionally adequate am[...]
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T. Wilkinson ; H. Ishikawa | 2001All phloem-feeding Homoptera possess symbiotic microorganisms. Although the phylogenetic position and anatomical location of the micro- organisms differ, the underlying theme of the symbiosis is the same; the microorganisms improve the nutrition[...]
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T. Wilkinson ; A. Douglas | 1998Plant penetration by the stylets of six clones of the pea aphid, Acyrthosiphon pisum, on Vicia faba (acceptable to all clones) and Pisum sativum (acceptable to 3/6 clones) was investigated by the DC electrical penetration graph technique. In a 1[...]
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D. Adams ; T. Wilkinson ; A. Douglas | 1996
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T. Wilkinson ; H. Ishikawa | 1999The failure of a nutritionally balanced diet to ameliorate the impact of symbiont disruption in the pea aphid Acyrthosiphon pisum (Harris) was investigated using two approaches. The assimilation of dietary nutrients by aphids was investigated us[...]
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T. Wilkinson | 1998Antibiotics are routinely used to eliminate intracellular prokaryotic microorganisms from a wide range of insect species, but concerns about deleterious effects of antibiotic therapy on the insect host are seldom addressed. Here, the impact of a[...]
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article/chapitre/communication
T. Wilkinson ; A. Douglas | 1995Aposgmbiotic pea aphids Acyrthosiphon pisum (i.e. aphids lacking functional symbiotic bacteria) were generated by treatment with the antibiotic rifampicin, Eight-day-old aposymbiotic aphids produced honeydew containing 16-30 mol% glutamine, whil[...]
