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Titre :
|
Transgenic potato plants with enhanced resistance to the peach-potato aphid Myzus persicae
|
|
Auteurs :
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A. Gatehouse ;
R. Down ;
K. Powell ;
N. Sauvion ;
Y. Rahbe ;
C. Newell ;
A. Merryweather ;
W. Hamilton ;
J. Gatehouse
|
|
Type de document :
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article/chapitre/communication
|
|
Année de publication :
|
1996
|
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Format :
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295-307
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Langues:
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= Anglais
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Mots-clés:
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Homoptera
;
Aphididae
;
lectins
;
transgenes
;
transgenic potato
;
resistance
;
Transfert de gene
;
Proteine
;
Fecondite
;
Taux de reproduction
;
Duree de developpement
;
Solanum tuberosum
;
Myzus persicae
|
|
Résumé :
|
Potato plants (Solanum tuberosum) cv. Desiree were transformed with the genes encoding the proteins bean chitinase (BCH), snowdrop lectin (GNA) and wheat a-amylase inhibitor (WAI) under the control of the constitutive Ca MV 35 S promoter. Transgenic plants with detectable levels of foreign RNA were then selected for further characterisation with respect to protein expression levels by immunodot blot analysis using polyclonal antibodies raised against the respective protein. With the exception of WAI, plants expressing high levels of RNA, expressed correspondingly high levels of the foreign protein (1.5-2.0% of the total soluble protein). Although high levels of WAI m RNA were detected in some of the transformants, the protein could not be detected. Both transgenic lines had a marked and significant effect on fecundity. Total nymphal production was also significantly lower on either of the transgenic lines compared to control plants (P
|
|
Source :
|
Entomologia Experimentalis et Applicata - 0013-8703, vol. 79, n° 3
|
