Titre :
|
Moderately increased constitutive proline does not alter osmotic stress tolerance
|
Auteurs :
|
A. Maggio ;
R. Bressan ;
P. Hasegawa ;
R. Locy
|
Type de document :
|
article/chapitre/communication
|
Année de publication :
|
1997
|
Format :
|
240-246
|
Langues:
|
= Anglais
|
Mots-clés:
|
carrot
;
Daucus carota
;
osmotic stress
;
proline
;
proline analogues
|
Résumé :
|
Proline-overproducing carrot cell lines were isolated by selection in medium containing hydroxyproline, a toxic analogue of proline, During growth of the cells in culture, length of lag phase, doubling time, and maximum fresh weight were the same for the hydroxyproline-resistant cell line (HP) and the wild-type cell line (SW). Proline content and resistance to hydroxyproline in the HP and JW lines were not strictly correlated indicating that another reason besides the constitutive level of proline is involved in hydroxyproline resistance. Tolerance to polyethylene glycol-induced desiccation stress was not different between the two lines except perhaps at the early stages of culture growth when the proline levels of the two cell lines were nearly the same. The complexity of the relationship between proline accumulation and osmotolerance is discussed and strategies to achieve constitutive high levels of proline accumulation in plants are proposed.
|
Source :
|
Physiologia Plantarum - 0031-9317, vol. 101, n° 1
|