Titre :
|
Specific detection of intracellular symbiotic bacteria of aphids by oligonucleotide-probed in situ hybridization
|
Auteurs :
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T. Fukatsu ;
K. Watanabe ;
Y. Sekiguchi
|
Type de document :
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article/chapitre/communication
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Année de publication :
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1998
|
Format :
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461-472
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Langues:
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= Anglais
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Mots-clés:
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aphid
;
intracellular symbiotic bacteria
;
primary and secondary symbionts
;
in situ hybridization
;
Symbionte
;
Acide nucleique
;
Marquage moleculaire
;
Acyrthosiphon pisum
;
Aphididae
;
Buchnera
|
Résumé :
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Oligonucleotide-probed in situ hybridization targeted to bacterial r RNAs was attempted to detect, visualize and characterize the intracellular symbiotic bacteria of aphids. Using the EUB338 probe that hybridizes universally with 16 S r RNA of eubacteria, the primary and secondary intracellular symbionts of various aphids were successfully stained on tissue thin sections. When in situ hybridization was conducted with GAM42 A and BET42 A probes that are targeted to 23 S r RNA of gamma- and P-subdivision of the Proteobacteria, respectively, it was shown that the secondary symbionts of Cinara pini and Nippolachnus piri belong to the gamma-subdivision of the Proteobacteria whereas that of Tetraneura radicicola is a member of the beta-subdivision of the Proteobacteria. This is the first report on the phylogenetic affinity of the secondary intracellular symbionts of aphids. Because insect tissues have strong autofluorescence in general, non-fluorescent in situ hybridization using biotin- and digoxigenin-labelled probes was more suitable for detecting symbiotic bacteria in aphid tissue sections.
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Source :
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Applied Entomology and Zoology - 0003-6862, vol. 33, n° 3
|