Titre : | Intracellular bacterial symbionts of aphids possess many genomic copies per bacterium |
Auteurs : | K. Komaki ; H. Ishikawa |
Type de document : | article/chapitre/communication |
Année de publication : | 1999 |
Format : | 717-722 |
Langues: | = Anglais |
Mots-clés: | Buchnera ; pea aphid ; symbiosis ; genomic copy number ; dot-blot analysis ; microscope photon-counting system ; polyploidy ; Symbionte ; Histologie ; Acide nucleique ; Acyrthosiphon pisum ; Aphididae |
Résumé : | Although Buchnera, the endosymbiotic bacteria of aphids, are close relatives of Escherichia coli, their genome size is only a seventh that of E. coli. In this study, we estimated the genomic copy number of Buchnera by dot-blot hybridization and fluorimetry using a video-intensified microscope photon-counting system and obtained convincing evidence that each cell of these bacteria contains an average of 120 genomic copies. Thus, the Buchnera symbiont, with many copies of a small-sized genome, is reminiscent of cell organelles such as mitochondria and chloroplasts. |
Source : | Journal of Molecular Evolution - 0022-2844, vol. 48, n° 6 |
Exemplaires (1)
Centre | Localisation | Section | Cote | Statut | Disponibilité |
---|---|---|---|---|---|
Poitou-Charentes | ERIST Poitou-Charentes - Lusignan | Base Aphide | A4C1E2 | Empruntable | Disponible pour le prêt |