Titre :
|
The effects of exopolymers on cell morphology and culturability of Leuconostoc mesenteroides during starvation
|
Auteurs :
|
D. Kim ;
H. Fogler
|
Type de document :
|
article/chapitre/communication
|
Année de publication :
|
1999
|
Format :
|
p. 839-844
|
Langues:
|
= Anglais
|
Catégories :
|
Biologie
|
Mots-clés:
|
ACTIVITE BACTERIENNE
;
PERMEABILITE
;
CELLULE
;
BIOMASSE
;
SOUS SOL
|
Résumé :
|
Biofilm formation by bacterial cells can be used to modify the subsurface permeability for the purpose of microbial enhanced oil recovery, bio-barrier formation, and in situ bioremediation. Once injected into the subsurface, the bacteria undergo starvation due to a decrease in nutrient supply and diffusion limitations in biofilms. To help understand the starvation response of bacteria in biofilms, the relationship between exopolymer formation and cell culturability was examined in a batch culture. The average cell diameter was observed to decrease from 0.8 m to 0.35 m 3 days after starvation began. Cell chain fragmentation was also observed during starvation. Cells that underwent starvation in the presence of insoluble exopolymers showed a slower rate of decrease in cell diameter and in cell chain length than cells without insoluble exopolymers. The rate of decrease in the average cell diameter and cell chain length were determined using a first order decay model.
|
Source :
|
Applied microbiology and biotechnology, vol.52, n°6
|