Titre :
|
Quantitative assessment of soil nitrate disappearance and N2O evolution during denitrification - Nitrate disappearance during denitrification
|
Auteurs :
|
S. Ellis ;
L. Dendooven ;
K. Goulding
|
Type de document :
|
article/chapitre/communication
|
Année de publication :
|
1996
|
Format :
|
p. 589-595
|
Langues:
|
= Anglais
|
Catégories :
|
CHIMIE
|
Mots-clés:
|
NITRATE
;
SOL
;
MICROBIOLOGIE
;
MATIERE ORGANIQUE
;
AMENDEMENT DU SOL
;
DENITRIFICATION
|
Résumé :
|
NO3- dynamics and evolution of N2O were investigated in a soil chemically-treated to control the amount and form of carbon, nitrogen and the microbial population. This was achieved by removing soil organic matter with hydrogen peroxide, leaving the soil inorganic skeleton. Treatments were : 1. Chemically-treated soil (low in C and N) amended with 500 mg NO3--N kg(-1) and 600 mg glucose-C kg(-1) (Control); 2. Chemically treated soil amended with 500 mg NO3--N kg(-1) and 600 mg glucose-C kg(-1) and a culture of Pseudomonas denitrificans, equivalent to 10(6) denitrifiers g(-1) of soil (Dentreat) and 3. Field soil amended with 500 mg NO3--N kg(-1) and 600 mg glucose-C kg(-1) and a culture of P. Denitrificans (Soil). Flasks were purged of O-2, shaken and anaerobically incubated with 10% C2H2 (v/v) for 96 h. The N2O and CO2 production and NO3- and NO2- concentrations were monitored. Approximately 10, 150 and 146 mg NO3--N kg(-1) disappeared from the soil solution in 2 h from the Control, Dentreat and Soil samples respectively. At the same time there was no concurrent increase in soil NO2- concentration or N2O evolution, whilst CO2 concentrations bore no stoichiometric relationship with N disappearance until the end of the incubation (96 h).
|
Source :
|
Soil biology and biochemistry, vol.28, n°4-5
|