Titre :
|
Respirometric assay for biofilm kinetics estimation: Parameter identifiability and retrievability
|
Auteurs :
|
R. Riefler ;
D. Ahlfeld ;
B. Smets
|
Type de document :
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article/chapitre/communication
|
Année de publication :
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1998
|
Format :
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p. 35-45
|
Langues:
|
= Anglais
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Catégories :
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Biologie
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Mots-clés:
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RESPIROMETRIE
;
CINETIQUE FERMENTAIRE
;
MODELE MATHEMATIQUE
|
Résumé :
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Currently, no fast and accurate methods exist for measuring extant biokinetic parameters for biofilm systems. This article presents a new approach to measure extant biokinetic parameters of biofilms and examines the numerical feasibility of such a method. A completely mixed attached growth bioreactor is subjected to a pulse of substrate, and oxygen consumption is monitored by on-line measurement of dissolved oxygen concentration in the bulk liquid. The oxygen concentration profile is then fit with a mechanistic mathematical model for the biofilm to estimate biokinetic parameters. In this study a transient biofilm model is developed and solved to generate dissolved oxygen profiles in the bulk liquid. Sensitivity analysis of the model reveals that the dissolved oxygen profiles are sufficiently sensitive to the biokinetic parameters-the maximum specific growth rate coefficient () and the half-saturation coefficient (K-s)-to support parameter estimation if accurate estimates of other model parameters can be obtained. Monte Carlo simulations are conducted with the model to add typical measurement error to the generated dissolved oxygen profiles.
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Source :
|
Biotechnology and Bioengineering, vol.57, n°1
|