Titre :
|
Rapid detection of Nocardia amarae in the activated sludge process using enzyme-linked immunosorbent assay (ELISA)
|
Auteurs :
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K. Iwahori ;
N. Miyata ;
S. Morisada ;
N. Suzuki
|
Type de document :
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article/chapitre/communication
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Année de publication :
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2000
|
Format :
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p. 469-473
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Langues:
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= Anglais
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Catégories :
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EAU
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Mots-clés:
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BOUES ACTIVEES
;
DETECTION
;
FOISONNEMENT DES BOUES
;
MICROORGANISME DE L'EPURATION
;
DYNAMIQUE DE POPULATION
|
Résumé :
|
Nocardia amarae, a mycolic acid-containing bacterium, has often been reported to cause foaming of activated sludge in wastewater treatment plants. In this study, the number of N. amarae cells in the activated sludge process was estimated by enzyme-linked immunosorbent assay (ELISA) with anti-N. amarae polyclonal antibody. Use of the antibody enabled N. amarae to be detected at levels of 10 to 10 colony forming units. On the other hand, the antibody reacted with only a small portion of activated sludge, in which no N. amarae cells were detected by the plate count method. Competitive ELISA was employed to estimate the N. amarae cells in samples taken from a municipal wastewater treatment plant, including raw wastewater and activated sludge foam. The cell numbers estimated by competitive ELISA corresponded well with those obtained by plate counts. Hence, the antibody produced in this study was shown to be effective for the rapid monitoring of N. amarae in the activated sludge process.
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Source :
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Journal of bioscience and bioengineering, vol.89, n°5
|